In Situ Observation of Antibiotic Susceptibility of Biofilms Using Microfluidic Device
نویسندگان
چکیده
Antibiotic susceptibility of biofilms is a growing public concern due to overuse use of antibiotics. Thus, determining an effective minimal concentration of antibiotics to eradicate biofilms is crucial. Here we present a novel one-pot assay for the analysis of antibiotic susceptibility of biofilms using a microfluidics system where continuous concentration gradients of antibiotics are generated. The results of minimal biofilm eradication concentration (MBEC) confirm that the concentration required to eradicate biofilm-grown Pseudomonas aeruginosa is higher than the minimal inhibitory concentration (MIC) that has been widely used to determine the lowest concentration of antibiotics against planktonically grown bacteria.
منابع مشابه
Microfluidics for Antibiotic Susceptibility and Toxicity Testing
The recent emergence of antimicrobial resistance has become a major concern for worldwide policy makers as very few new antibiotics have been developed in the last twenty-five years. To prevent the death of millions of people worldwide, there is an urgent need for a cheap, fast and accurate set of tools and techniques that can help to discover and develop new antimicrobial drugs. In the past de...
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